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  • SuperScript® II Reverse Transcriptase
  • 型号:18064-014
  • 报价:
  • 地区:上海市
  • 0
  • SuperScript® II Reverse Transcriptase 18064-014 现货促销,价格超优!更多优惠!更多现货,欢迎免费订购: :、、
  • 025-58690567
产品详细介绍
货号

单位规格

目录价格 (CNY)促销价
18064-01410,000 units

3,918.00

询价
18064-0222,000 units

1,142.00

询价
18064-0714 x 10,000 units

13,860.00

询价

SuperScript? II Reverse Transcriptase 18064-014 

描述

SuperScript? II Reverse Transcriptase (RT) is an improved version of SuperScript? RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript? II RT superior performance characteristics.

Yield – Reduced RNase H activity results in greater first-strand cDNA yields
Size of cDNA – Generation of RT-PCR products up to 12 kb
Thermostability – Full activity at 42°C
Flexibility – Uses either total or poly(A)+ RNA

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.

Unit Definition: One unit of SuperScript? II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (3).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

SuperScript? II Reverse Transcriptase 18064-014 

规格

Optimal Reaction Temperature:42° C
Final Product Size(s):12.3 kb or less
Reverse Transcriptase:SuperScript? II
Ribonuclease H Activity:Reduced
Form:Frozen
Format:Tube(s)
Quantity:2000 units
Template:ssDNA, ssRNA, RNA-DNA Hybrid
Sensitivity:Medium
Product Size:2,000 units
Concentration:200 U?μl
Enzyme Function:RNA dependent DNA Polymerase
Shipping Condition:Wet Ice
Number of Reactions:10 Reactions

SuperScript? II Reverse Transcriptase 18064-014 

内容及储存

SuperScript? II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored.

For Research Use Only. Not for use in diagnostic procedures.

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