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  • LC3B抗体,LC3B/MAP1LC3B Antibody Autophagosomes
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  • LC3B抗体,LC3B/MAP1LC3B Antibody Autophagosomes Marker,Novus经典抗体,300多篇引用文献 更多的现货产品,免费订购: :、、
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LC3B/MAP1LC3B Antibody Summary

SpeciesHuman, Mouse, Rat, Bacteria, Bovine, Golden Syrian Hamster,Porcine, Primate, Zebrafish
Tested ApplicationsWB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality

Polyclonal

Host

Rabbit

Gene

MAP1LC3B

Purity

Immunogen affinity purified

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LC3B/MAP1LC3B Antibody Details

Marker

Autophagosomes Marker

Immunogen

A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). [UniProt# Q9GZQ8]

Localization

LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.

Species Reactivity

Human, rat, mouse, zebrafish, bacteria, hamster, and porcine. Bovine reactivity reported in scientific literature (PMID: 24895572) Primate reactivity reported in scientific literature (PMID: 25142602) The mouse detection has been reported to be weaker than the human. Immunogen sequence has 84% homology to Xenopus.

Reviewed SpeciesRead 20 rated 4.6  using NB100-2220 in the following species:
 
  • Human

    8 reviews

  • Mouse

    7 reviews

  • Rat

    2 reviews

  • Other

    2 reviews

PublicationsRead Publications using NB100-2220 in the following species:
 
  • Human

    123 Publications

  • Mouse

    93 Publications

  • Rat

    22 Publications

  • Bacteria

    1 Publication

  • Bovine

    2 Publications

  • Golden Syrian Hamster

    1 Publication

  • Hamster

    1 Publication

  • Porcine

    4 Publications

  • Primate

    1 Publication

  • Zebrafish

    1 Publication

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Immunoprecipitation 20ug/500ug of protein
Application Notes

This LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. Use in Immunohistochemistry-Frozen sections reported in scientific literature (PMID: 20008275) In Western blot bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at ~21 kDa in mouse protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells.In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Positive Controls

Positive Controls

 

Reviewed Applications
Read 20 Review rated 4.6  using 
NB100-2220 in the following applications:
 
  • Western Blot

    17

  • Immunohistochemistry

    1

  • Immunohistochemistry-Paraffin

    1

  • Immunoprecipitation

    1

Publications
Read Publications using
NB100-2220 in the following applications:
 
  • WB

    210 publications

  • FLOW

    1 publication

  • ICC/IF

    46 publications

  • IHC

    17 publications

  • IHC-Fr

    6 publications

  • IHC-P

    8 publications

  • IP

    1 publication


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